Principle of the assay: sCD36 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-sCD36 antibody and an sCD36-HRP conjugate. The assay sample and buffer are incubated together with sCD36-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sCD36 concentration since sCD36 from samples and sCD36-HRP conjugate compete for the anti-sCD36 antibody binding site. Since the number of sites is limited, as more sites are occupied by sCD36 from the sample, fewer sites are left to bind sCD36-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD36 concentration in each sample is interpolated from this standard curve.FREE 8GB USB Flash Drive with the purchase of this item.
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