Microtiter wells coated with recombinant HBsAg (adw and adr), are exposed to test specimens, which may contain Rabbit IgG reactive antibodies. After an incubation period, unbound components in the test sample are washed away. Specific bound Rabbit IgG react with an anti-Rabbit IgG conjugated with HRP during a second incubation period. Following a second wash cycle, specific bound enzyme conjugate is detected by reaction with the substrate solution, 2,2' Azino-di[3-ethyl-benzthiazoline­sulfonate (ABTS). The assay is measured spectrophotometrically to indicate the level of Rabbit Anti-HbsAg antibodies present in a sample. *FOR RESEARCH USE ONLY*
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