Stellar Scientific - High Purity Plasmid Miniprep Kit with 100 spin columns

Description

Stellar Scientific™s High Purity Plasmid Miniprep Kit is designed for rapid and cost-effective small-scale preparation of high quality plasmid DNA from recombinant E.coli cultures. The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. Each spin column can recover up to 40μg of plasmid DNA. The kit can be successfully used for efficient purification of any size plasmids and cosmids. The actual plasmid yield and optimal culture volume depend on the plasmid copy number and medium used for cultivation. Applications Purified DNA is free from contaminants and enzyme inhibitors, and typically has A260/A280 ratios between 1.7 and 1.9, and is suitable for applications such as:, Restriction digestion, PCR, DNA Sequencing, in vitro transcription Features, Fast - procedure takes only 30 min., Efficient “ can get 10-40µg high copy plasmid DNA in one prep., High purity - Purified DNA is ready for downstream application such as PCR, restriction digestion Kit Contents RNase A 300 μl Resuspension Solution 30 ml Lysis Solution 30 ml Neutralization Solution 40 ml Wash Buffer PB 60 ml Wash Buffer W 30 ml×2 Elution Buffer (10 mM Tris-HCl, pH 8.5) 10 ml Spin Columns 100 each