ImmunoTek Plus™ Stop Reagent, Microwell Substrate, HRP (405nm, 650nm) Microwell Substrate is a proprietary liquid reagent specifically designed to stop the Microwell Substrate Stop Solution reaction. Microwell Substrate is tailored for Microwell Substrate Stop Solution. A soluble blue reaction develops that can be stopped for end point readings at either 405nm or 650nm using Microwell Substrate Stop Solution. The Microwell Peroxidase Substrate will produce kinetic signal when read at 405nm, the recommended wavelength, about 40% higher than conventional. Reading at 650nm will provide an additional 40% increase in kinetic signal compared to 405nm readings. Microwell Substrate Stop Solution will stop the substrate reaction and provide end point readings at either wavelength. The end point readings will be stable for at least an hour. Intended Use: Microwell Substrate is specifically designed to terminate the peroxidase reaction. Equal volumes of substrate and Microwell Substrate solution should be used. When Microwell Substrate peroxidase substrate solution is reacted with peroxidase, a reaction ensues producing a blue colored soluble liquid product that can be read at 405nm or 650nm. Microwell Substrate Stop Solution stops the reaction for end point readings at 405nm or 650nm. The end point is stable for at least an hour. Storage and Stability: Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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